› 4ogz

X-ray diffraction
2Å resolution

Crystal structure of a putative alpha-galactosidase/melibiase (BF4189) from Bacteroides fragilis NCTC 9343 at 2.00 A resolution

Released:
DOI: 10.2210/pdb4ogz/pdb
PDB entry 4ogz coloured by chain, front view.
PDB entry 4ogz coloured by chain, side view.
PDB entry 4ogz coloured by chain, top view.
Source organism: Bacteroides fragilis NCTC 9343
Entry author: Joint Center for Structural Genomics (JCSG)

Function and Biology Details

Reaction catalysed: 
Hydrolysis of terminal, non-reducing alpha-D-galactose residues in alpha-D-galactosides, including galactose oligosaccharides, galactomannans and galactolipids
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assemblies composition:
monomeric
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-177440 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Alpha-galactosidase Chains: A, B
Molecule details ›
Chains: A, B
Length: 473 amino acids
Theoretical weight: 53.12 KDa
Source organism: Bacteroides fragilis NCTC 9343
Expression system: Escherichia coli
UniProt:
  • Canonical: Q5L7T7 (Residues: 25-496; Coverage: 100%)
Gene name: BF9343_4081
Sequence domains:
Structure domains:

Ligands and Environments

3 bound ligands:
Ligand PGE 2 x PGE
Ligand PEG 8 x PEG
Ligand PO4 1 x PO4
1 modified residue:
Ligand MSE 20 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: ALS BEAMLINE 8.2.1
Spacegroup: P21
Unit cell:
a: 50.955Å b: 170.874Å c: 52.619Å
α: 90° β: 106.71° γ: 90°
R-values:
R R work R free
0.171 0.169 0.201
Expression system: Escherichia coli

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