› 4oh1

X-ray diffraction
2Å resolution

Crystal structure of a putative zinc-binding dehydrogenase (gutB) from Clostridium scindens ATCC 35704 at 2.00 A resolution

Released:
DOI: 10.2210/pdb4oh1/pdb
PDB entry 4oh1 coloured by chain, front view.
PDB entry 4oh1 coloured by chain, side view.
PDB entry 4oh1 coloured by chain, top view.
Source organism: [Clostridium] scindens ATCC 35704
Entry author: Joint Center for Structural Genomics (JCSG)

Function and Biology Details

Reaction catalysed: 
L-iditol + NAD(+) = L-sorbose + NADH
Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-109188 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Sorbitol dehydrogenase Chain: A
Molecule details ›
Chain: A
Length: 351 amino acids
Theoretical weight: 39.67 KDa
Source organism: [Clostridium] scindens ATCC 35704
Expression system: Escherichia coli
UniProt:
  • Canonical: B0NC68 (Residues: 1-350; Coverage: 100%)
Gene names: HDCHBGLK_00843, gutB_2
Sequence domains:
Structure domains:

Ligands and Environments

4 bound ligands:
Ligand ZN 2 x ZN
Ligand CL 2 x CL
Ligand EDO 1 x EDO
Ligand PG4 1 x PG4
2 modified residues:
Ligand MSE 20 x MSE
Ligand OCS 1 x OCS

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRL BEAMLINE BL14-1
Spacegroup: I222
Unit cell:
a: 63.87Å b: 85.597Å c: 149.087Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.17 0.168 0.216
Expression system: Escherichia coli

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