› 4oif

X-ray diffraction
2.45Å resolution

3D structure of Gan42B, a GH42 beta-galactosidase from G.

Released:
DOI: 10.2210/pdb4oif/pdb
Model geometry
Fit model/data
PDB entry 4oif coloured by chain, front view.
PDB entry 4oif coloured by chain, side view.
PDB entry 4oif coloured by chain, top view.
Source organism: Geobacillus stearothermophilus
Primary publication:
Crystallization and preliminary crystallographic analysis of GanB, a GH42 intracellular β-galactosidase from Geobacillus stearothermophilus.
Solomon HV, Tabachnikov O, Feinberg H, Govada L, Chayen NE, Shoham Y, Shoham G
Acta Crystallogr Sect F Struct Biol Cryst Commun 69 1114-9 (2013)
PMID: 24100561

Function and Biology Details

Reaction catalysed: 
Hydrolysis of terminal non-reducing beta-D-galactose residues in beta-D-galactosides
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo trimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-123730 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Beta-galactosidase Chains: A, B, C
Molecule details ›
Chains: A, B, C
Length: 686 amino acids
Theoretical weight: 80.1 KDa
Source organism: Geobacillus stearothermophilus
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: F8TRX0 (Residues: 1-686; Coverage: 100%)
Gene name: ganB
Sequence domains:
Structure domains:

Ligands and Environments

2 bound ligands:
Ligand ZN 3 x ZN
Ligand GOL 25 x GOL
No modified residues

Experiments and Validation Details

wwPDB Validation report is not available for this entry.
X-ray source: ESRF BEAMLINE ID14-1
Spacegroup: P212121
Unit cell:
a: 71.84Å b: 181.35Å c: 196.57Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.136 0.133 0.204
Expression system: Escherichia coli BL21

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Citations

6 citation in other articles

Genomic analysis of six new Geobacillus strains reveals highly conserved carbohydrate degradation architectures and strategies.
Brumm et al. (2015)
5 more

4 mentions without citation

Structural Insights into the Molecular Evolution of the Archaeal Exo-β-d-Glucosaminidase.
Mine et al. (2019)
3 more