› 4ojv

X-ray diffraction
1.31Å resolution

Function and Biology Details

Reaction catalysed: 
Nucleoside 3',5'-cyclic phosphate + H(2)O = nucleoside 5'-phosphate
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-149630 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
3',5'-cyclic-nucleotide phosphodiesterase 1 Chain: A
Molecule details ›
Chain: A
Length: 369 amino acids
Theoretical weight: 42.07 KDa
Source organism: Saccharomyces cerevisiae S288C
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: P22434 (Residues: 1-369; Coverage: 100%)
Gene names: NRB369, PDE1, YGL248W
Sequence domains: cAMP phosphodiesterases class-II

Ligands and Environments

3 bound ligands:
Ligand SO4 1 x SO4
Ligand MPD 1 x MPD
Ligand ZN 2 x ZN
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X29A
Spacegroup: C2221
Unit cell:
a: 73.478Å b: 85.151Å c: 130.565Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.173 0.172 0.184
Expression system: Escherichia coli BL21

Quick links

Citations

4 review citations

In Search of Enzymes with a Role in 3', 5'-Cyclic Guanosine Monophosphate Metabolism in Plants.
Gross et al. (2016)
3 more

3 mentions without citation

Dual specificity and novel structural folding of yeast phosphodiesterase-1 for hydrolysis of second messengers cyclic adenosine and guanosine 3',5'-monophosphate.
Tian et al. (2014)
2 more