› 4ok7

X-ray diffraction
1.9Å resolution

Structure of bacteriophage SPN1S endolysin from Salmonella typhimurium

Released:
DOI: 10.2210/pdb4ok7/pdb
PDB entry 4ok7 coloured by chain, front view.
PDB entry 4ok7 coloured by chain, side view.
PDB entry 4ok7 coloured by chain, top view.
Source organism: Salmonella phage SPN1S
Primary publication:
Structure of bacteriophage SPN1S endolysin reveals an unusual two-module fold for the peptidoglycan lytic and binding activity.
Park Y, Lim JA, Kong M, Ryu S, Rhee S
Mol Microbiol 92 316-25 (2014)
PMID: 24641441

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assemblies composition:
homo trimer
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-124742 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Glycoside hydrolase family 19 catalytic domain-containing protein Chains: A, B, C
Molecule details ›
Chains: A, B, C
Length: 223 amino acids
Theoretical weight: 24.94 KDa
Source organism: Salmonella phage SPN1S
Expression system: Escherichia coli
UniProt:
  • Canonical: H2D0G4 (Residues: 1-208; Coverage: 100%)
Gene name: SPN1S_0028
Sequence domains: Chitinase class I

Ligands and Environments

2 bound ligands:
Ligand SO4 14 x SO4
Ligand GOL 4 x GOL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: PHOTON FACTORY BEAMLINE BL-1A
Spacegroup: P212121
Unit cell:
a: 54.616Å b: 120.597Å c: 126.474Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.208 0.208 0.236
Expression system: Escherichia coli

Quick links

Citations

5 review citations

Breaking barriers: expansion of the use of endolysins as novel antibacterials against Gram-negative bacteria.
Briers et al. (2015)
4 more

6 mentions without citation

Endolysins from Antarctic Pseudomonas Display Lysozyme Activity at Low Temperature.
Orlando et al. (2020)
5 more