› 4oln

X-ray diffraction
1.7Å resolution

Ancestral Steroid Receptor 1 in complex with estrogen response element DNA

Released:
DOI: 10.2210/pdb4oln/pdb
PDB entry 4oln coloured by chain, front view.
PDB entry 4oln coloured by chain, side view.
PDB entry 4oln coloured by chain, top view.
Source organism: synthetic construct
Primary publication:
Evolution of DNA specificity in a transcription factor family produced a new gene regulatory module.
McKeown AN, Bridgham JT, Anderson DW, Murphy MN, Ortlund EA, Thornton JW
Cell 159 58-68 (2014)
PMID: 25259920

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
PDBe Complex ID:
PDB-CPX-117923 (preferred)
Entry contents:
1 distinct polypeptide molecule
2 distinct DNA molecules
Macromolecules (3 distinct):
AncSR1 Chains: A, B
Molecule details ›
Chains: A, B
Length: 82 amino acids
Theoretical weight: 9.37 KDa
Source organism: synthetic construct
Expression system: Escherichia coli BL21
Structure domains: Erythroid Transcription Factor GATA-1, subunit A
5'-D(*CP*CP*AP*GP*GP*TP*CP*AP*GP*AP*GP*TP*GP*AP*CP*CP*TP*GP*A)-3' Chain: E
Molecule details ›
Chain: E
Length: 19 nucleotides
Theoretical weight: 5.85 KDa
Source organism: synthetic construct
Expression system: Not provided
5'-D(*TP*CP*AP*GP*GP*TP*CP*AP*CP*TP*CP*TP*GP*AP*CP*CP*TP*GP*G)-3' Chain: F
Molecule details ›
Chain: F
Length: 19 nucleotides
Theoretical weight: 5.8 KDa
Source organism: synthetic construct
Expression system: Not provided

Ligands and Environments

2 bound ligands:
Ligand ZN 4 x ZN
Ligand NA 1 x NA
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 22-ID
Spacegroup: C2
Unit cell:
a: 97.184Å b: 36.425Å c: 90.939Å
α: 90° β: 121.63° γ: 90°
R-values:
R R work R free
0.176 0.175 0.206
Expression systems:
  • Escherichia coli BL21
  • Not provided

Quick links

Citations

15 review citations

Epistasis in protein evolution.
Starr et al. (2016)
14 more

4 mentions without citation

Alternative evolutionary histories in the sequence space of an ancient protein.
Starr et al. (2017)
3 more