› 4olt

X-ray diffraction
1.59Å resolution

Chitosanase complex structure

Released:
DOI: 10.2210/pdb4olt/pdb
PDB entry 4olt coloured by chain, front view.
PDB entry 4olt coloured by chain, side view.
PDB entry 4olt coloured by chain, top view.
Source organism: Pseudomonas sp. LL2(2010)
Primary publication:
Structural insights into the substrate-binding mechanism for a novel chitosanase.
Lyu Q, Wang S, Xu W, Han B, Liu W, Jones DN, Liu W
Biochem J 461 335-45 (2014)
PMID: 24766439

Function and Biology Details

Reaction catalysed: 
Endohydrolysis of beta-(1->4)-linkages between D-glucosamine residues in a partly acetylated chitosan
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-122743 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecules (2 distinct):
Chitosanase Chains: A, B
Molecule details ›
Chains: A, B
Length: 248 amino acids
Theoretical weight: 26.61 KDa
Source organism: Pseudomonas sp. LL2(2010)
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: E1AXU1 (Residues: 27-266; Coverage: 100%)
Gene name: CHI
Sequence domains: Glycosyl hydrolase family 46
Structure domains:

Ligands and Environments

Carbohydrate polymer : NEW Components: GCS
Carbohydrate polymer
1 bound ligand:
Ligand GOL 3 x GOL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRF BEAMLINE BL17U
Spacegroup: P21
Unit cell:
a: 62.023Å b: 40.705Å c: 104.794Å
α: 90° β: 106.53° γ: 90°
R-values:
R R work R free
0.164 0.161 0.226
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

3 review citations

Interaction between chitosan and its related enzymes: A review.
Shinya et al. (2017)
2 more

5 mentions without citation

Chitosanases from Family 46 of Glycoside Hydrolases: From Proteins to Phenotypes.
Viens et al. (2015)
4 more