› 4omj

X-ray diffraction
1.6Å resolution

Crystal structure of SPF bound to 2,3-oxidosqualene

Released:
DOI: 10.2210/pdb4omj/pdb
PDB entry 4omj coloured by chain, front view.
PDB entry 4omj coloured by chain, side view.
PDB entry 4omj coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Structural insights on cholesterol endosynthesis: Binding of squalene and 2,3-oxidosqualene to supernatant protein factor.
Christen M, Marcaida MJ, Lamprakis C, Aeschimann W, Vaithilingam J, Schneider P, Hilbert M, Schneider G, Cascella M, Stocker A
J Struct Biol 190 261-70 (2015)
PMID: 25987292

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-131192 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
SEC14-like protein 2 Chains: A, B
Molecule details ›
Chains: A, B
Length: 278 amino acids
Theoretical weight: 31.99 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: O76054 (Residues: 1-275; Coverage: 68%)
Gene names: C22orf6, KIAA1186, KIAA1658, SEC14L2
Sequence domains:
Structure domains: CRAL-TRIO lipid binding domain

Ligands and Environments

3 bound ligands:
Ligand SO4 7 x SO4
Ligand CL 9 x CL
Ligand 2TX 2 x 2TX
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SLS BEAMLINE X06DA
Spacegroup: P21
Unit cell:
a: 71.88Å b: 73.8Å c: 94.45Å
α: 90° β: 96.29° γ: 90°
R-values:
R R work R free
0.156 0.154 0.179
Expression system: Escherichia coli

Quick links

Citations

3 review citations

Current Insights into the Biological Action of Squalene.
Lou-Bonafonte et al. (2018)
2 more