› 4ond

X-ray diffraction
2.25Å resolution

Ancestral Steroid Receptor 2 DBD helix mutant - ERE DNA complex

Released:
DOI: 10.2210/pdb4ond/pdb
PDB entry 4ond coloured by chain, front view.
PDB entry 4ond coloured by chain, side view.
PDB entry 4ond coloured by chain, top view.
Source organism: synthetic construct
Primary publication:
Evolution of DNA specificity in a transcription factor family produced a new gene regulatory module.
McKeown AN, Bridgham JT, Anderson DW, Murphy MN, Ortlund EA, Thornton JW
Cell 159 58-68 (2014)
PMID: 25259920

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
PDBe Complex ID:
PDB-CPX-117926 (preferred)
Entry contents:
1 distinct polypeptide molecule
2 distinct DNA molecules
Macromolecules (3 distinct):
Ancestral SR2 Helix Mutant Chains: A, B, E, F
Molecule details ›
Chains: A, B, E, F
Length: 82 amino acids
Theoretical weight: 9.05 KDa
Source organism: synthetic construct
Expression system: Escherichia coli
Structure domains: Erythroid Transcription Factor GATA-1, subunit A
5'-D(*CP*CP*AP*GP*GP*TP*CP*AP*GP*AP*GP*TP*GP*AP*CP*CP*TP*G)-3' Chains: I, K
Molecule details ›
Chains: I, K
Length: 18 nucleotides
Theoretical weight: 5.54 KDa
Source organism: synthetic construct
Expression system: Not provided
5'-D(*TP*CP*AP*GP*GP*TP*CP*AP*CP*TP*CP*TP*GP*AP*CP*CP*TP*G)-3' Chains: J, L
Molecule details ›
Chains: J, L
Length: 18 nucleotides
Theoretical weight: 5.47 KDa
Source organism: synthetic construct
Expression system: Not provided

Ligands and Environments

1 bound ligand:
Ligand ZN 8 x ZN
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 22-ID
Spacegroup: P21
Unit cell:
a: 48.272Å b: 79.829Å c: 116.782Å
α: 90° β: 96.75° γ: 90°
R-values:
R R work R free
0.188 0.186 0.216
Expression systems:
  • Escherichia coli
  • Not provided

Quick links

Citations

15 review citations

Epistasis in protein evolution.
Starr et al. (2016)
14 more

2 mentions without citation

Alternative evolutionary histories in the sequence space of an ancient protein.
Starr et al. (2017)
1 more