› 4oo9

X-ray diffraction
2.6Å resolution

Structure of the human class C GPCR metabotropic glutamate receptor 5 transmembrane domain in complex with the negative allosteric modulator mavoglurant

Released:
DOI: 10.2210/pdb4oo9/pdb
PDB entry 4oo9 coloured by chain, front view.
PDB entry 4oo9 coloured by chain, side view.
PDB entry 4oo9 coloured by chain, top view.
Source organisms:
Primary publication:
Structure of class C GPCR metabotropic glutamate receptor 5 transmembrane domain.
Doré AS, Okrasa K, Patel JC, Serrano-Vega M, Bennett K, Cooke RM, Errey JC, Jazayeri A, Khan S, Tehan B, Weir M, Wiggin GR, Marshall FH
Nature 511 557-62 (2014)
PMID: 25042998

Function and Biology Details

Reaction catalysed: 
Hydrolysis of (1->4)-beta-linkages between N-acetylmuramic acid and N-acetyl-D-glucosamine residues in a peptidoglycan and between N-acetyl-D-glucosamine residues in chitodextrins
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-132987 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Endolysin; Metabotropic glutamate receptor 5 Chain: A
Molecule details ›
Chain: A
Length: 444 amino acids
Theoretical weight: 49.92 KDa
Source organisms: Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: P41594 (Residues: 569-678, 680-836; Coverage: 22%)
  • Canonical: P00720 (Residues: 2-162; Coverage: 98%)
Gene names: E, GPRC1E, GRM5, MGLUR5
Sequence domains:
Structure domains: Lysozyme

Ligands and Environments

3 bound ligands:
Ligand OLA 4 x OLA
Ligand 2U8 1 x 2U8
Ligand MES 1 x MES
1 modified residue:
Ligand YCM 2 x YCM

Experiments and Validation Details

Entry percentile scores
X-ray source: DIAMOND BEAMLINE I24
Spacegroup: C2
Unit cell:
a: 143.242Å b: 43.555Å c: 82.049Å
α: 90° β: 99.37° γ: 90°
R-values:
R R work R free
0.241 0.239 0.275
Expression system: Spodoptera frugiperda

Quick links

Citations

72 review citations

Generic GPCR residue numbers - aligning topology maps while minding the gaps.
Isberg et al. (2015)
71 more

53 mentions without citation

A comprehensive review of the lipid cubic phase or in meso method for crystallizing membrane and soluble proteins and complexes.
Caffrey M. (2015)
52 more