› 4opc

X-ray diffraction
1.4Å resolution

Constructing tailored isoprenoid products by structure-guided modification of geranylgeranyl reductase.

Released:
DOI: 10.2210/pdb4opc/pdb
PDB entry 4opc coloured by chain, front view.
PDB entry 4opc coloured by chain, side view.
PDB entry 4opc coloured by chain, top view.
Source organism: Sulfolobus acidocaldarius DSM 639
Primary publication:
Constructing tailored isoprenoid products by structure-guided modification of geranylgeranyl reductase.
Kung Y, McAndrew RP, Xie X, Liu CC, Pereira JH, Adams PD, Keasling JD
Structure 22 1028-36 (2014)
PMID: 24954619

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-175571 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Digeranylgeranylglycerophospholipid reductase Chain: A
Molecule details ›
Chain: A
Length: 453 amino acids
Theoretical weight: 50.83 KDa
Source organism: Sulfolobus acidocaldarius DSM 639
Expression system: Escherichia coli
UniProt:
  • Canonical: Q4JA33 (Residues: 1-452; Coverage: 100%)
Gene name: Saci_0986
Sequence domains: FAD dependent oxidoreductase
Structure domains: FAD/NAD(P)-binding domain

Ligands and Environments

2 bound ligands:
Ligand FDA 1 x FDA
Ligand PGT 1 x PGT
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ALS BEAMLINE 8.2.1
Spacegroup: C2
Unit cell:
a: 108.524Å b: 65.253Å c: 63.203Å
α: 90° β: 91.98° γ: 90°
R-values:
R R work R free
0.152 0.151 0.178
Expression system: Escherichia coli

Quick links

Citations

7 review citations

Natural products as biofuels and bio-based chemicals: fatty acids and isoprenoids.
Beller et al. (2015)
6 more

1 mention without citation

Self-association of a highly charged arginine-rich cell-penetrating peptide.
Tesei et al. (2017)