› 4opd

X-ray diffraction
1.81Å resolution

Constructing tailored isoprenoid products by structure-guided modification of geranylgeranyl reductase.

Released:
DOI: 10.2210/pdb4opd/pdb
PDB entry 4opd coloured by chain, front view.
PDB entry 4opd coloured by chain, side view.
PDB entry 4opd coloured by chain, top view.
Source organism: Sulfolobus acidocaldarius DSM 639
Primary publication:
Constructing tailored isoprenoid products by structure-guided modification of geranylgeranyl reductase.
Kung Y, McAndrew RP, Xie X, Liu CC, Pereira JH, Adams PD, Keasling JD
Structure 22 1028-36 (2014)
PMID: 24954619

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-175571 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Digeranylgeranylglycerophospholipid reductase Chains: A, B
Molecule details ›
Chains: A, B
Length: 453 amino acids
Theoretical weight: 50.83 KDa
Source organism: Sulfolobus acidocaldarius DSM 639
Expression system: Escherichia coli
UniProt:
  • Canonical: Q4JA33 (Residues: 1-452; Coverage: 100%)
Gene name: Saci_0986
Sequence domains: FAD dependent oxidoreductase
Structure domains: FAD/NAD(P)-binding domain

Ligands and Environments

2 bound ligands:
Ligand FDA 2 x FDA
Ligand GRG 6 x GRG
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ALS BEAMLINE 8.2.1
Spacegroup: P1
Unit cell:
a: 63.079Å b: 63.234Å c: 65.102Å
α: 120.98° β: 89.97° γ: 88.55°
R-values:
R R work R free
0.146 0.146 0.191
Expression system: Escherichia coli

Quick links

Citations

6 review citations

Natural products as biofuels and bio-based chemicals: fatty acids and isoprenoids.
Beller et al. (2015)
5 more

5 mentions without citation

Oxygen Activation by Cu LPMOs in Recalcitrant Carbohydrate Polysaccharide Conversion to Monomer Sugars.
Meier et al. (2018)
4 more