› 4or1

X-ray diffraction
3Å resolution

Structure and mechanism of fibronectin binding and biofilm formation of enteroaggregative Escherischia coli AAF fimbriae

Released:
DOI: 10.2210/pdb4or1/pdb
PDB entry 4or1 coloured by chain, front view.
PDB entry 4or1 coloured by chain, side view.
PDB entry 4or1 coloured by chain, top view.
Source organism: Escherichia coli
Primary publication:
Structural insight into host recognition by aggregative adherence fimbriae of enteroaggregative Escherichia coli.
Berry AA, Yang Y, Pakharukova N, Garnett JA, Lee WC, Cota E, Marchant J, Roy S, Tuittila M, Liu B, Inman KG, Ruiz-Perez F, Mandomando I, Nataro JP, Zavialov AV, Matthews S
PLoS Pathog 10 e1004404 (2014)
PMID: 25232738

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-128255 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Invasin homolog AafB; Major fimbrial subunit of aggregative adherence fimbria II AafA Chains: A, B
Molecule details ›
Chains: A, B
Length: 155 amino acids
Theoretical weight: 17.19 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli
UniProt:
  • Canonical: Q9X4L4 (Residues: 12-12, 24-146; Coverage: 91%)
  • Canonical: O30595 (Residues: 26-35; Coverage: 7%)
Gene names: aafA, aafB
Sequence domains: Enterobacteria AfaD invasin protein
Structure domains: Dr adhesin

Ligands and Environments

2 bound ligands:
Ligand SO4 1 x SO4
Ligand ACT 1 x ACT
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: DIAMOND BEAMLINE I24
Spacegroup: P6122
Unit cell:
a: 81.755Å b: 81.755Å c: 222.703Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.221 0.22 0.248
Expression system: Escherichia coli

Quick links

Citations

6 review citations

Classical chaperone-usher (CU) adhesive fimbriome: uropathogenic Escherichia coli (UPEC) and urinary tract infections (UTIs).
Behzadi P. (2020)
5 more

5 mentions without citation

Interaction with the host: the role of fibronectin and extracellular matrix proteins in the adhesion of Gram-negative bacteria.
Vaca et al. (2020)
4 more