4osh

X-ray diffraction
2.2Å resolution

Crystal structure of the TAL effector dHax3 with NI RVD at 2.2 angstrom resolution

Released:
Primary publication:
Revisiting the TALE repeat.
Protein Cell 5 297-306 (2014)
PMID: 24622844

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domain:

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
PDBe Complex ID:
PDB-CPX-117934 (preferred)
Entry contents:
1 distinct polypeptide molecule
2 distinct DNA molecules
Macromolecules (3 distinct):
Avirulence protein AvrBs3 Chains: A, B
Molecule details ›
Chains: A, B
Length: 499 amino acids
Theoretical weight: 51.78 KDa
Source organism: Xanthomonas campestris pv. armoraciae
Expression system: Escherichia coli
UniProt:
  • Canonical: Q3ZD72 (Residues: 231-720; Coverage: 51%)
Gene name: hax3
Sequence domains: TAL effector repeat
DNA (5'-D(*TP*GP*TP*CP*CP*CP*TP*TP*TP*AP*TP*CP*TP*CP*TP*CP*T)-3') Chains: G, I
Molecule details ›
Chains: G, I
Length: 17 nucleotides
Theoretical weight: 5.07 KDa
Source organism: Xanthomonas campestris pv. armoraciae
Expression system: Not provided
DNA (5'-D(*AP*GP*AP*GP*AP*GP*AP*TP*AP*AP*AP*GP*GP*GP*AP*CP*A)-3') Chains: H, J
Molecule details ›
Chains: H, J
Length: 17 nucleotides
Theoretical weight: 5.34 KDa
Source organism: Xanthomonas campestris pv. armoraciae
Expression system: Not provided

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRF BEAMLINE BL17U
Spacegroup: P21
Unit cell:
a: 84.529Å b: 80.987Å c: 89.083Å
α: 90° β: 103.62° γ: 90°
R-values:
R R work R free
0.207 0.205 0.246
Expression systems:
  • Escherichia coli
  • Not provided