› 4osk

X-ray diffraction
2.4Å resolution

Crystal structure of TAL effector reveals the recognition between asparagine and guanine

Released:
DOI: 10.2210/pdb4osk/pdb
PDB entry 4osk coloured by chain, front view.
PDB entry 4osk coloured by chain, side view.
PDB entry 4osk coloured by chain, top view.
Source organism: Xanthomonas campestris pv. armoraciae
Primary publication:
Revisiting the TALE repeat.
Deng D, Yan C, Wu J, Pan X, Yan N
Protein Cell 5 297-306 (2014)
PMID: 24622844

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domain:

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-117937 (preferred)
Entry contents:
1 distinct polypeptide molecule
2 distinct DNA molecules
Macromolecules (3 distinct):
Avirulence protein AvrBs3 Chains: A, B
Molecule details ›
Chains: A, B
Length: 499 amino acids
Theoretical weight: 51.85 KDa
Source organism: Xanthomonas campestris pv. armoraciae
Expression system: Escherichia coli
UniProt:
  • Canonical: Q3ZD72 (Residues: 231-720; Coverage: 51%)
Gene name: hax3
Sequence domains: TAL effector repeat
DNA (5'-D(*TP*GP*TP*CP*CP*AP*AP*CP*TP*AP*CP*TP*AP*GP*AP*CP*T)-3') Chains: G, I
Molecule details ›
Chains: G, I
Length: 17 nucleotides
Theoretical weight: 5.15 KDa
Source organism: Xanthomonas campestris pv. armoraciae
Expression system: Not provided
DNA (5'-D(*AP*GP*TP*CP*TP*AP*GP*TP*AP*GP*TP*TP*GP*GP*AP*CP*A)-3') Chains: H, J
Molecule details ›
Chains: H, J
Length: 17 nucleotides
Theoretical weight: 5.27 KDa
Source organism: Xanthomonas campestris pv. armoraciae
Expression system: Not provided

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRF BEAMLINE BL17U
Spacegroup: P21
Unit cell:
a: 84.633Å b: 88.129Å c: 90.476Å
α: 90° β: 104.96° γ: 90°
R-values:
R R work R free
0.216 0.214 0.255
Expression systems:
  • Escherichia coli
  • Not provided

Quick links

Citations

2 review citations

Strategies for precision modulation of gene expression by epigenome editing: an overview.
Laufer et al. (2015)
1 more