› 4otc

X-ray diffraction
2.28Å resolution

4-OXALOCROTONATE TAUTOMERASE OBSERVED AS AN OCTODECAMER, TRIGONAL CRYSTAL FORM

Released:
DOI: 10.2210/pdb4otc/pdb
Model geometry
Data not deposited
Fit model/data
Data not deposited
PDB entry 4otc coloured by chain, front view.
PDB entry 4otc coloured by chain, side view.
PDB entry 4otc coloured by chain, top view.
Source organism: Pseudomonas putida
Primary publication:
Native and inhibitor complex structures of 4-oxalocrotonate tautomerase from Pseudomonas putida mt-2 (University of Texas at Austin-136 pages)
Taylor AB
Thesis (1998)

Function and Biology Details

Reaction catalysed: 
(2Z,4E)-2-hydroxyhexa-2,4-dienedioate = (3E)-2-oxohex-3-enedioate
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:
Structure domain:

Structure analysis Details

Assemblies composition:
homo hexamer
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-169563 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
2-hydroxymuconate tautomerase Chains: A, B, C, D, E, F, G, H, I
Molecule details ›
Chains: A, B, C, D, E, F, G, H, I
Length: 62 amino acids
Theoretical weight: 6.82 KDa
Source organism: Pseudomonas putida
Expression system: Escherichia coli
UniProt:
  • Canonical: Q01468 (Residues: 2-63; Coverage: 98%)
Gene name: xylH
Sequence domains: Tautomerase enzyme
Structure domains: Macrophage Migration Inhibitory Factor

Ligands and Environments

1 bound ligand:
Ligand SO4 18 x SO4
No modified residues

Experiments and Validation Details

wwPDB Validation report is not available for this entry.
X-ray source: RIGAKU RU200
Spacegroup: P321
Unit cell:
a: 88Å b: 88Å c: 124.6Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.234 0.234 0.265
Expression system: Escherichia coli

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Citations

2 mentions without citation

Global and local structural similarity in protein-protein complexes: implications for template-based docking.
Kundrotas et al. (2013)
1 more