4ou7

X-ray diffraction
2.83Å resolution

Crystal structure of DnaT84-153-dT10 ssDNA complex reveals a novel single-stranded DNA binding mode

Released:
Model geometry
Fit model/data

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domain:

Structure analysis Details

Assembly composition:
hetero hexamer (preferred)
PDBe Complex ID:
PDB-CPX-117951 (preferred)
Entry contents:
1 distinct polypeptide molecule
1 distinct DNA molecule
Macromolecules (2 distinct):
Replication restart protein DnaT Chains: A, B, C, D, E
Molecule details ›
Chains: A, B, C, D, E
Length: 71 amino acids
Theoretical weight: 8.17 KDa
Source organism: Escherichia coli K-12
Expression system: Escherichia coli
UniProt:
  • Canonical: P0A8J2 (Residues: 84-154; Coverage: 40%)
Gene names: JW4326, b4362, dnaT
Sequence domains: DnaT DNA-binding domain
Structure domains: Helicase, Ruva Protein; domain 3
DNA (5'-D(P*TP*TP*TP*TP*TP*TP*TP*TP*TP*T)-3') Chain: S
Molecule details ›
Chain: S
Length: 10 nucleotides
Theoretical weight: 3 KDa
Source organism: Escherichia coli K-12
Expression system: Not provided

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

wwPDB Validation report is not available for this entry.
X-ray source: SSRF BEAMLINE BL17U
Spacegroup: P1
Unit cell:
a: 46.408Å b: 46.689Å c: 54.392Å
α: 87.33° β: 86.01° γ: 70.2°
R-values:
R R work R free
0.186 0.183 0.238
Expression systems:
  • Escherichia coli
  • Not provided