› 4ovw

X-ray diffraction
2.3Å resolution

ENDOGLUCANASE I COMPLEXED WITH EPOXYBUTYL CELLOBIOSE

Released:
DOI: 10.2210/pdb4ovw/pdb
PDB entry 4ovw coloured by chain, front view.
PDB entry 4ovw coloured by chain, side view.
PDB entry 4ovw coloured by chain, top view.
Source organism: Fusarium oxysporum
Primary publication:
Structure of the endoglucanase I from Fusarium oxysporum: native, cellobiose, and 3,4-epoxybutyl beta-D-cellobioside-inhibited forms, at 2.3 A resolution.
Sulzenbacher G, Schülein M, Davies GJ
Biochemistry 36 5902-11 (1997)
PMID: 9153432

Function and Biology Details

Reaction catalysed: 
Endohydrolysis of (1->4)-beta-D-glucosidic linkages in cellulose, lichenin and cereal beta-D-glucans
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-155450 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Endoglucanase type C Chains: A, B
Molecule details ›
Chains: A, B
Length: 411 amino acids
Theoretical weight: 44.69 KDa
Source organism: Fusarium oxysporum
UniProt:
  • Canonical: P46237 (Residues: 19-429; Coverage: 100%)
Sequence domains: Glycosyl hydrolase family 7
Structure domains: Glycoside hydrolase, family 7, domain

Ligands and Environments

2 bound ligands:
Ligand NAG 2 x NAG
Ligand IN1 2 x IN1
1 modified residue:
Ligand PCA 2 x PCA

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU FR-C
Spacegroup: P21
Unit cell:
a: 65.94Å b: 82.62Å c: 73.16Å
α: 90° β: 94.16° γ: 90°
R-values:
R R work R free
0.18 0.18 0.27

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Citations

5 review citations

Structural and sequence-based classification of glycoside hydrolases.
Henrissat et al. (1997)
4 more

2 mentions without citation

Indole-Containing Phytoalexin-Based Bioisosteres as Antifungals: In Vitro and In Silico Evaluation against Fusarium oxysporum.
Angarita-Rodríguez et al. (2019)
1 more