› 4oxv

X-ray diffraction
2.2Å resolution

Crystal structure of MltF from Pseudomonas aeruginosa complexed with valine

Released:
DOI: 10.2210/pdb4oxv/pdb
PDB entry 4oxv coloured by chain, front view.
PDB entry 4oxv coloured by chain, side view.
PDB entry 4oxv coloured by chain, top view.
Source organism: Pseudomonas aeruginosa PADK2_CF510
Entry authors: Reddem E, Thunnissen AMWH

Function and Biology Details

Reaction catalysed: 
Exolytic cleavage of the (1->4)-beta-glycosidic linkage between N-acetylmuramic acid (MurNAc) and N-acetylglucosamine (GlcNAc) residues in peptidoglycan, from either the reducing or the non-reducing ends of the peptidoglycan chains, with concomitant formation of a 1,6-anhydrobond in the MurNAc residue.
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-102244 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Membrane-bound lytic murein transglycosylase F Chain: A
Molecule details ›
Chain: A
Length: 435 amino acids
Theoretical weight: 49.29 KDa
Source organism: Pseudomonas aeruginosa PADK2_CF510
Expression system: Escherichia coli
UniProt:
  • Canonical: A0A0J9X1Z1 (Residues: 1-435; Coverage: 100%)
Gene names: CF510_16633, mltF
Sequence domains:
Structure domains: Periplasmic binding protein-like II

Ligands and Environments

1 bound ligand:
Ligand VAL 1 x VAL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: BRUKER AXS MICROSTAR
Spacegroup: P212121
Unit cell:
a: 52.881Å b: 80.671Å c: 89.219Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.179 0.176 0.244
Expression system: Escherichia coli

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Citations

2 mentions without citation

Crystal Structures of the SpoIID Lytic Transglycosylases Essential for Bacterial Sporulation.
Nocadello et al. (2016)
1 more