› 4oz0

X-ray diffraction
2.2Å resolution

Crystal structure of human CAPERalpha U2AF homology motif (apo-state)

Released:
DOI: 10.2210/pdb4oz0/pdb
PDB entry 4oz0 coloured by chain, front view.
PDB entry 4oz0 coloured by chain, side view.
PDB entry 4oz0 coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Cancer-relevant splicing factor CAPERα engages the essential splicing factor SF3b155 in a specific ternary complex.
Loerch S, Maucuer A, Manceau V, Green MR, Kielkopf CL
J Biol Chem 289 17325-37 (2014)
PMID: 24795046

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-172019 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
RNA-binding protein 39 Chains: A, B
Molecule details ›
Chains: A, B
Length: 115 amino acids
Theoretical weight: 12.66 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: Q14498 (Residues: 417-530; Coverage: 22%)
Gene names: HCC1, RBM39, RNPC2
Structure domains: Alpha-Beta Plaits

Ligands and Environments

1 bound ligand:
Ligand CL 1 x CL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: OTHER
Spacegroup: P212121
Unit cell:
a: 44.653Å b: 52.41Å c: 85.141Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.198 0.196 0.223
Expression system: Escherichia coli BL21

Quick links

Citations

10 review citations

Altered RNA Processing in Cancer Pathogenesis and Therapy.
Obeng et al. (2019)
9 more

3 mentions without citation

Cancer-relevant splicing factor CAPERα engages the essential splicing factor SF3b155 in a specific ternary complex.
Loerch et al. (2014)
2 more