› 4ozt

X-ray diffraction
2.7Å resolution

crystal structure of the ligand binding domains of the Bovicola ovis ecdysone receptor EcR/USP heterodimer (PonA crystal)

Released:
DOI: 10.2210/pdb4ozt/pdb
Model geometry
Fit model/data
PDB entry 4ozt coloured by chain, front view.
PDB entry 4ozt coloured by chain, side view.
PDB entry 4ozt coloured by chain, top view.
Source organism: Pediculus humanus corporis
Primary publication:
Unprecedented conformational flexibility revealed in the ligand-binding domains of the Bovicola ovis ecdysone receptor (EcR) and ultraspiracle (USP) subunits.
Ren B, Peat TS, Streltsov VA, Pollard M, Fernley R, Grusovin J, Seabrook S, Pilling P, Phan T, Lu L, Lovrecz GO, Graham LD, Hill RJ
Acta Crystallogr D Biol Crystallogr 70 1954-64 (2014)
PMID: 25004972

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-122706 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Ecdysone receptor Chain: E
Molecule details ›
Chain: E
Length: 238 amino acids
Theoretical weight: 27.48 KDa
Source organism: Pediculus humanus corporis
Expression system: Escherichia coli
UniProt:
  • Canonical: E0VVT4 (Residues: 281-518; Coverage: 46%)
Gene names: 8238728, Phum_PHUM467460
Sequence domains: Ligand-binding domain of nuclear hormone receptor
Structure domains: Retinoid X Receptor
Nuclear receptor subfamily 2 group B member 4 Chain: U
Molecule details ›
Chain: U
Length: 195 amino acids
Theoretical weight: 21.98 KDa
Source organism: Pediculus humanus corporis
Expression system: Escherichia coli
UniProt:
  • Canonical: E0VFQ5 (Residues: 222-416; Coverage: 46%)
Gene names: 8236603, Phum_PHUM164330
Sequence domains: Ligand-binding domain of nuclear hormone receptor
Structure domains: Retinoid X Receptor

Ligands and Environments

2 bound ligands:
Ligand P1A 1 x P1A
Ligand NEQ 1 x NEQ
No modified residues

Experiments and Validation Details

wwPDB Validation report is not available for this entry.
X-ray source: AUSTRALIAN SYNCHROTRON BEAMLINE MX1
Spacegroup: P43212
Unit cell:
a: 134Å b: 134Å c: 95.3Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.184 0.182 0.217
Expression system: Escherichia coli

Quick links

Citations

8 citation in other articles

Genetic Evidence for Function of the bHLH-PAS Protein Gce/Met As a Juvenile Hormone Receptor.
Jindra et al. (2015)
7 more

1 mention without citation

In Silico Prediction of the Mechanism of Action of Pyriproxyfen and 4'-OH-Pyriproxyfen against A. mellifera and H. sapiens Receptors.
Spaggiari et al. (2021)