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› 4ozy

X-ray diffraction
2.9Å resolution

Crystal Structure of the periplasmic alginate epimerase AlgG T265N mutant

Released:
DOI: 10.2210/pdb4ozy/pdb
Model geometry
Fit model/data
PDB entry 4ozy coloured by chain, front view.
PDB entry 4ozy coloured by chain, side view.
PDB entry 4ozy coloured by chain, top view.
Source organism: Pseudomonas syringae pv. tomato str. DC3000
Entry authors: Howell PL, Wolfram F, Robinson H

Function and Biology Details

Reaction catalysed: 
[(1->4)-beta-D-mannuronosyl](n) = [alginate](n).
Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assemblies composition:
monomeric (preferred)
homo dimer
Assembly name:
PDBe Complex ID:
PDB-CPX-183429 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Mannuronan C5-epimerase Chain: A
Molecule details ›
Chain: A
Length: 423 amino acids
Theoretical weight: 47.5 KDa
Source organism: Pseudomonas syringae pv. tomato str. DC3000
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q887Q3 (Residues: 69-491; Coverage: 85%)
Gene names: PSPTO_1238, algG
Sequence domains: Right handed beta helix region
Structure domains: Single-stranded right-handed beta-helix, Pectin lyase-like

Ligands and Environments

No bound ligands
1 modified residue:
Ligand MLY 3 x MLY

Experiments and Validation Details

wwPDB Validation report is not available for this entry.
X-ray source: NSLS BEAMLINE X29A
Spacegroup: P3221
Unit cell:
a: 124.782Å b: 124.782Å c: 98.031Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.18 0.176 0.221
Expression system: Escherichia coli BL21(DE3)

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