› 4p1q

X-ray diffraction
1.5Å resolution

GREEN FLUORESCENT PROTEIN E222H VARIANT

Released:
DOI: 10.2210/pdb4p1q/pdb
PDB entry 4p1q coloured by chain, front view.
PDB entry 4p1q coloured by chain, side view.
PDB entry 4p1q coloured by chain, top view.
Source organism: Aequorea victoria
Primary publication:
Replacement of highly conserved E222 by the photostable non-photoconvertible histidine in GFP.
Auerbach D, Klein M, Franz S, Carius Y, Lancaster CR, Jung G
Chembiochem 15 1404-8 (2014)
PMID: 24919579

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-154537 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Green fluorescent protein Chain: A
Molecule details ›
Chain: A
Length: 228 amino acids
Theoretical weight: 25.82 KDa
Source organism: Aequorea victoria
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P42212 (Residues: 3-231; Coverage: 96%)
Gene name: GFP
Sequence domains: Green fluorescent protein
Structure domains: Green fluorescent protein

Ligands and Environments

1 bound ligand:
Ligand NA 1 x NA
1 modified residue:
Ligand GYS 1 x GYS

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID14-4
Spacegroup: P212121
Unit cell:
a: 50.6Å b: 62.2Å c: 67.57Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.138 0.136 0.172
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

4 citation in other articles

Structural Insight into the Photochemistry of Split Green Fluorescent Proteins: A Unique Role for a His-Tag.
Deng et al. (2018)
3 more

1 mention without citation

Atomic insights into the genesis of cellular filaments by globular proteins.
McPartland et al. (2018)