› 4p29

X-ray diffraction
1.95Å resolution

Crystal structure of the LpoA N-terminal domain from Haemophilus influenzae

Released:
DOI: 10.2210/pdb4p29/pdb
PDB entry 4p29 coloured by chain, front view.
PDB entry 4p29 coloured by chain, side view.
PDB entry 4p29 coloured by chain, top view.
Source organism: Haemophilus influenzae Rd KW20
Primary publication:
Structural analyses of the Haemophilus influenzae peptidoglycan synthase activator LpoA suggest multiple conformations in solution.
Sathiyamoorthy K, Vijayalakshmi J, Tirupati B, Fan L, Saper MA
J Biol Chem 292 17626-17642 (2017)
PMID: 28887305

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-155267 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Penicillin-binding protein activator LpoA Chains: A, B
Molecule details ›
Chains: A, B
Length: 231 amino acids
Theoretical weight: 26.4 KDa
Source organism: Haemophilus influenzae Rd KW20
Expression system: Escherichia coli
UniProt:
  • Canonical: P45299 (Residues: 33-253; Coverage: 40%)
Gene names: HI_1655, lpoA
Sequence domains: LppC putative lipoprotein
Structure domains: Serine Threonine Protein Phosphatase 5, Tetratricopeptide repeat

Ligands and Environments

3 bound ligands:
Ligand SO4 3 x SO4
Ligand GOL 2 x GOL
Ligand CL 1 x CL
1 modified residue:
Ligand MSE 6 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 5ID-B
Spacegroup: P212121
Unit cell:
a: 48.03Å b: 51.18Å c: 198.61Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.186 0.184 0.218
Expression system: Escherichia coli

Quick links

Citations

1 review citation

Normal Mode Analysis as a Routine Part of a Structural Investigation.
Bauer et al. (2019)
6 other articles

2 mentions without citation

Structural analyses of the Haemophilus influenzae peptidoglycan synthase activator LpoA suggest multiple conformations in solution.
Sathiyamoorthy et al. (2017)
1 more