› 4p2x

X-ray diffraction
2.4Å resolution

Swapped Dimer of Mycobacterial Adenylyl cyclase Rv1625c: Form 2

Released:
DOI: 10.2210/pdb4p2x/pdb
PDB entry 4p2x coloured by chain, front view.
PDB entry 4p2x coloured by chain, side view.
PDB entry 4p2x coloured by chain, top view.
Source organism: Mycobacterium tuberculosis
Primary publication:
New structural forms of a mycobacterial adenylyl cyclase Rv1625c.
Barathy D, Mattoo R, Visweswariah S, Suguna K
IUCrJ 1 338-48 (2014)
PMID: 25295175

Function and Biology Details

Reaction catalysed: 
ATP = 3',5'-cyclic AMP + diphosphate
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-162043 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Adenylate cyclase Chains: A, B
Molecule details ›
Chains: A, B
Length: 257 amino acids
Theoretical weight: 28.61 KDa
Source organism: Mycobacterium tuberculosis
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: P9WQ35 (Residues: 212-443; Coverage: 52%)
Gene names: MTCY01B2.17c, Rv1625c, cya
Sequence domains: Adenylate and Guanylate cyclase catalytic domain
Structure domains: Nucleotide cyclase

Ligands and Environments

4 bound ligands:
Ligand SO4 1 x SO4
Ligand PEG 4 x PEG
Ligand MG 1 x MG
Ligand CL 1 x CL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE BM14
Spacegroup: P65
Unit cell:
a: 75.57Å b: 75.57Å c: 133.17Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.199 0.196 0.26
Expression system: Escherichia coli BL21

Quick links

Citations

5 citation in other articles

Role of the nucleotidyl cyclase helical domain in catalytically active dimer formation.
Vercellino et al. (2017)
4 more

1 mention without citation

New structural forms of a mycobacterial adenylyl cyclase Rv1625c.
Barathy et al. (2014)