› 4p35

X-ray diffraction
1.75Å resolution

Crystal structure of DJ-1 with Zinc(II) bound (crystal I)

Released:
DOI: 10.2210/pdb4p35/pdb
PDB entry 4p35 coloured by chain, front view.
PDB entry 4p35 coloured by chain, side view.
PDB entry 4p35 coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Thermodynamic and structural characterization of the specific binding of Zn(II) to human protein DJ-1.
Tashiro S, Caaveiro JM, Wu CX, Hoang QQ, Tsumoto K
Biochemistry 53 2218-20 (2014)
PMID: 24697266

Function and Biology Details

Reaction catalysed: 
An N(6)-(1-hydroxy-2-oxopropyl)-[protein]-L-lysine + H(2)O = a [protein]-L-lysine + lactate
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-189207 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Parkinson disease protein 7 Chain: A
Molecule details ›
Chain: A
Length: 189 amino acids
Theoretical weight: 19.92 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: Q99497 (Residues: 1-189; Coverage: 100%)
Gene name: PARK7
Sequence domains: DJ-1/PfpI family
Structure domains: Rossmann fold

Ligands and Environments

3 bound ligands:
Ligand ZN 1 x ZN
Ligand CL 1 x CL
Ligand GOL 1 x GOL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: PHOTON FACTORY BEAMLINE BL-5A
Spacegroup: P3121
Unit cell:
a: 75.13Å b: 75.13Å c: 75.54Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.143 0.142 0.171
Expression system: Escherichia coli BL21

Quick links

Citations

3 review citations

Parkinson's disease: Alterations in iron and redox biology as a key to unlock therapeutic strategies.
Ma et al. (2021)
2 more

1 mention without citation

A palindrome-driven complex rearrangement of 22q11.2 and 8q24.1 elucidated using novel technologies.
Gotter et al. (2007)