› 4p3g

X-ray diffraction
2.7Å resolution

Structure of the SRP68-RBD from Chaetomium thermophilum

Released:
DOI: 10.2210/pdb4p3g/pdb
PDB entry 4p3g coloured by chain, front view.
PDB entry 4p3g coloured by chain, side view.
PDB entry 4p3g coloured by chain, top view.
Source organism: Chaetomium thermophilum var. thermophilum DSM 1495
Primary publication:
SRP RNA remodeling by SRP68 explains its role in protein translocation.
Grotwinkel JT, Wild K, Segnitz B, Sinning I
Science 344 101-4 (2014)
PMID: 24700861

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-124016 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Signal recognition particle subunit SRP68 Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 224 amino acids
Theoretical weight: 25.1 KDa
Source organism: Chaetomium thermophilum var. thermophilum DSM 1495
Expression system: Escherichia coli
UniProt:
  • Canonical: G0S5V2 (Residues: 2-217; Coverage: 34%)
Gene name: CTHT_0033730
Sequence domains: RNA-binding signal recognition particle 68
Structure domains: Signal recognition particle, SRP68 subunit, RNA-binding domain

Ligands and Environments

1 bound ligand:
Ligand PO4 5 x PO4
1 modified residue:
Ligand MSE 20 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID23-1
Spacegroup: P212121
Unit cell:
a: 46.84Å b: 95.05Å c: 223.859Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.202 0.2 0.253
Expression system: Escherichia coli

Quick links

Citations

6 review citations

SRPassing Co-translational Targeting: The Role of the Signal Recognition Particle in Protein Targeting and mRNA Protection.
Kellogg et al. (2021)
5 more