› 4p6j

X-ray diffraction
2.8Å resolution

Crystal Structure of the Computationally Designed Transmembrane Metallotransporter with 4-bromophenylalanine in Octyl Glucoside

Released:
DOI: 10.2210/pdb4p6j/pdb
PDB entry 4p6j coloured by chain, front view.
PDB entry 4p6j coloured by chain, side view.
PDB entry 4p6j coloured by chain, top view.
Source organism: synthetic construct
Primary publication:
De novo design of a transmembrane Zn²⁺-transporting four-helix bundle.
Joh NH, Wang T, Bhate MP, Acharya R, Wu Y, Grabe M, Hong M, Grigoryan G, DeGrado WF
Science 346 1520-4 (2014)
PMID: 25525248

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-164985 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Computationally Designed Transporter of Zn(II) and Proton Chains: A, B
Molecule details ›
Chains: A, B
Length: 26 amino acids
Theoretical weight: 3.02 KDa
Source organism: synthetic construct
Expression system: Not provided

Ligands and Environments

1 bound ligand:
Ligand SO4 1 x SO4
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ALS BEAMLINE 8.3.1
Spacegroup: P43212
Unit cell:
a: 41.45Å b: 41.45Å c: 63.99Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.249 0.248 0.272
Expression system: Not provided

Quick links

Citations

47 review citations

The coming of age of de novo protein design.
Huang et al. (2016)
46 more

2 mentions without citation

De novo design of a transmembrane Zn²⁺-transporting four-helix bundle.
Joh et al. (2014)
1 more