› 4p79

X-ray diffraction
2.4Å resolution

Crystal structure of mouse claudin-15

Released:
DOI: 10.2210/pdb4p79/pdb
PDB entry 4p79 coloured by chain, front view.
PDB entry 4p79 coloured by chain, side view.
PDB entry 4p79 coloured by chain, top view.
Source organism: Mus musculus
Primary publication:
Crystal structure of a claudin provides insight into the architecture of tight junctions.
Suzuki H, Nishizawa T, Tani K, Yamazaki Y, Tamura A, Ishitani R, Dohmae N, Tsukita S, Nureki O, Fujiyoshi Y
Science 344 304-7 (2014)
PMID: 24744376

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-195529 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Claudin-15 Chain: A
Molecule details ›
Chain: A
Length: 198 amino acids
Theoretical weight: 21.44 KDa
Source organism: Mus musculus
Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: Q9Z0S5 (Residues: 2-194; Coverage: 85%)
Gene name: Cldn15
Sequence domains: PMP-22/EMP/MP20/Claudin family
Structure domains: Butyryl-CoA Dehydrogenase, subunit A; domain 3

Ligands and Environments

1 bound ligand:
Ligand OLC 2 x OLC
1 modified residue:
Ligand MSE 8 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: SPRING-8 BEAMLINE BL32XU
Spacegroup: C2
Unit cell:
a: 123.25Å b: 28.403Å c: 58.587Å
α: 90° β: 100.84° γ: 90°
R-values:
R R work R free
0.224 0.221 0.253
Expression system: Spodoptera frugiperda

Quick links

Citations

68 review citations

Tight junctions: from simple barriers to multifunctional molecular gates.
Zihni et al. (2016)
67 more

32 mentions without citation

A comprehensive review of the lipid cubic phase or in meso method for crystallizing membrane and soluble proteins and complexes.
Caffrey M. (2015)
31 more