› 4p7b

X-ray diffraction
1.6Å resolution

Crystal structure of S. typhimurium peptidyl-tRNA hydrolase

Released:
DOI: 10.2210/pdb4p7b/pdb
PDB entry 4p7b coloured by chain, front view.
PDB entry 4p7b coloured by chain, side view.
PDB entry 4p7b coloured by chain, top view.
Source organism: Salmonella enterica subsp. enterica serovar Typhimurium str. LT2
Primary publication:
Recombinant production, crystallization and X-ray crystallographic structure determination of peptidyl-tRNA hydrolase from Salmonella typhimurium.
Vandavasi V, Taylor-Creel K, McFeeters RL, Coates L, McFeeters H
Acta Crystallogr F Struct Biol Commun 70 872-7 (2014)
PMID: 25005080

Function and Biology Details

Reaction catalysed: 
N-substituted aminoacyl-tRNA + H(2)O = N-substituted amino acid + tRNA
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-101269 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Peptidyl-tRNA hydrolase Chains: A, C
Molecule details ›
Chains: A, C
Length: 200 amino acids
Theoretical weight: 22.04 KDa
Source organism: Salmonella enterica subsp. enterica serovar Typhimurium str. LT2
Expression system: Escherichia coli
UniProt:
  • Canonical: A0A0F6B281 (Residues: 2-194; Coverage: 100%)
Gene names: STM14_2156, pth
Sequence domains: Peptidyl-tRNA hydrolase
Structure domains: Peptidyl-tRNA hydrolase

Ligands and Environments

1 bound ligand:
Ligand TRS 2 x TRS
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 19-ID
Spacegroup: P212121
Unit cell:
a: 62.097Å b: 64.925Å c: 110.489Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.175 0.174 0.198
Expression system: Escherichia coli

Quick links

Citations

6 citation in other articles

Activity of acetyltransferase toxins involved in Salmonella persister formation during macrophage infection.
Rycroft et al. (2018)
5 more

3 mentions without citation

Recombinant production, crystallization and X-ray crystallographic structure determination of peptidyl-tRNA hydrolase from Salmonella typhimurium.
Vandavasi et al. (2014)
2 more