› 4p86

X-ray diffraction
1.93Å resolution

Structure of PyrR protein from Bacillus subtilis with GMP

Released:
DOI: 10.2210/pdb4p86/pdb
PDB entry 4p86 coloured by chain, front view.
PDB entry 4p86 coloured by chain, side view.
PDB entry 4p86 coloured by chain, top view.
Source organism: Bacillus subtilis
Primary publication:
Evolution of oligomeric state through allosteric pathways that mimic ligand binding.
Perica T, Kondo Y, Tiwari SP, McLaughlin SH, Kemplen KR, Zhang X, Steward A, Reuter N, Clarke J, Teichmann SA
Science 346 1254346 (2014)
PMID: 25525255

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-164994 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Bifunctional protein PyrR Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 183 amino acids
Theoretical weight: 20.43 KDa
Source organism: Bacillus subtilis
Expression system: Escherichia coli BL21(DE3)
Structure domains: Rossmann fold

Ligands and Environments

2 bound ligands:
Ligand 5GP 6 x 5GP
Ligand GOL 1 x GOL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: DIAMOND BEAMLINE I02
Spacegroup: C2
Unit cell:
a: 97.94Å b: 77.53Å c: 99.78Å
α: 90° β: 102.05° γ: 90°
R-values:
R R work R free
0.181 0.18 0.214
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

11 review citations

Phosphoribosyl Diphosphate (PRPP): Biosynthesis, Enzymology, Utilization, and Metabolic Significance.
Hove-Jensen et al. (2017)
10 more

1 mention without citation

Evolution of oligomeric state through allosteric pathways that mimic ligand binding.
Perica et al. (2014)