› 4p8b

X-ray diffraction
1.3Å resolution

CRYSTAL STRUCTURE OF A TRAP PERIPLASMIC SOLUTE BINDING PROTEIN FROM RALSTONIA EUTROPHA H16 (H16_A1328), TARGET EFI-510189, WITH BOUND (S)-2-hydroxy-2-methyl-3-oxobutanoate ((S)-2-Acetolactate)

Released:
DOI: 10.2210/pdb4p8b/pdb
Model geometry
Fit model/data
PDB entry 4p8b coloured by chain, front view.
PDB entry 4p8b coloured by chain, side view.
PDB entry 4p8b coloured by chain, top view.
Source organism: Cupriavidus necator H16
Primary publication:
Experimental strategies for functional annotation and metabolism discovery: targeted screening of solute binding proteins and unbiased panning of metabolomes.
Vetting MW, Al-Obaidi N, Zhao S, San Francisco B, Kim J, Wichelecki DJ, Bouvier JT, Solbiati JO, Vu H, Zhang X, Rodionov DA, Love JD, Hillerich BS, Seidel RD, Quinn RJ, Osterman AL, Cronan JE, Jacobson MP, Gerlt JA, Almo SC
OpenAccess logo Biochemistry 54 909-31 (2015)
PMID: 25540822

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-170980 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
TRAP-type transporter, periplasmic component Chain: A
Molecule details ›
Chain: A
Length: 342 amino acids
Theoretical weight: 37.7 KDa
Source organism: Cupriavidus necator H16
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q0KC03 (Residues: 1-342; Coverage: 100%)
Gene names: E6A55_06705, H16_A1328
Sequence domains: Bacterial extracellular solute-binding protein, family 7
Structure domains: Bacterial extracellular solute-binding protein, family 7

Ligands and Environments

2 bound ligands:
Ligand CL 2 x CL
Ligand X2X 1 x X2X
1 modified residue:
Ligand MSE 10 x MSE

Experiments and Validation Details

wwPDB Validation report is not available for this entry.
X-ray source: APS BEAMLINE 31-ID
Spacegroup: R3
Unit cell:
a: 140.193Å b: 140.193Å c: 38.579Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.155 0.154 0.174
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

14 review citations

Enzyme Function Initiative-Enzyme Similarity Tool (EFI-EST): A web tool for generating protein sequence similarity networks.
Gerlt et al. (2015)
13 more

1 mention without citation

Experimental strategies for functional annotation and metabolism discovery: targeted screening of solute binding proteins and unbiased panning of metabolomes.
Vetting et al. (2015)