› 4p9g

X-ray diffraction
2.2Å resolution

Structure of the 2,4'-dihydroxyacetophenone dioxygenase from Alcaligenes sp.

Released:
DOI: 10.2210/pdb4p9g/pdb
PDB entry 4p9g coloured by chain, front view.
PDB entry 4p9g coloured by chain, side view.
PDB entry 4p9g coloured by chain, top view.
Source organism: Alcaligenes sp.
Primary publication:
Structure of the 2,4'-dihydroxyacetophenone dioxygenase from Alcaligenes sp. 4HAP.
Keegan R, Lebedev A, Erskine P, Guo J, Wood SP, Hopper DJ, Rigby SE, Cooper JB
Acta Crystallogr D Biol Crystallogr 70 2444-54 (2014)
PMID: 25195757

Function and Biology Details

Reaction catalysed: 
2,4'-dihydroxyacetophenone + O(2) = 4-hydroxybenzoate + formate
Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-193155 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Cupin type-2 domain-containing protein Chain: A
Molecule details ›
Chain: A
Length: 197 amino acids
Theoretical weight: 22.69 KDa
Source organism: Alcaligenes sp.
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q9REI7 (Residues: 2-176; Coverage: 99%)
Gene name: dad
Sequence domains: Cupin domain
Structure domains: Jelly Rolls

Ligands and Environments

3 bound ligands:
Ligand FE 1 x FE
Ligand CO3 1 x CO3
Ligand GOL 1 x GOL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE BM14
Spacegroup: P6522
Unit cell:
a: 82.557Å b: 82.557Å c: 114.008Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.176 0.173 0.218
Expression system: Escherichia coli BL21(DE3)

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Citations

3 citation in other articles

The Polycyclic Aromatic Hydrocarbon (PAH) degradation activities and genome analysis of a novel strain Stenotrophomonas sp. Pemsol isolated from Mexico.
Elufisan et al. (2020)
2 more

3 mentions without citation

Ensembles generated from crystal structures of single distant homologues solve challenging molecular-replacement cases in AMPLE.
Rigden et al. (2018)
2 more