› 4pbg

X-ray diffraction
2.5Å resolution

6-PHOSPHO-BETA-GALACTOSIDASE FORM-CST

Released:
DOI: 10.2210/pdb4pbg/pdb
PDB entry 4pbg coloured by chain, front view.
PDB entry 4pbg coloured by chain, side view.
PDB entry 4pbg coloured by chain, top view.
Source organism: Lactococcus lactis
Primary publication:
Crystal structures and mechanism of 6-phospho-beta-galactosidase from Lactococcus lactis.
Wiesmann C, Hengstenberg W, Schulz GE
J Mol Biol 269 851-60 (1997)
PMID: 9223646

Function and Biology Details

Reaction catalysed: 
A 6-phospho-beta-D-galactoside + H(2)O = 6-phospho-D-galactose + an alcohol
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-145873 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
6-phospho-beta-galactosidase Chains: A, B
Molecule details ›
Chains: A, B
Length: 468 amino acids
Theoretical weight: 54.11 KDa
Source organism: Lactococcus lactis
Expression system: Escherichia coli
UniProt:
  • Canonical: P11546 (Residues: 1-468; Coverage: 100%)
Gene name: lacG
Sequence domains: Glycosyl hydrolase family 1
Structure domains: Glycosidases

Ligands and Environments

1 bound ligand:
Ligand BGP 2 x BGP
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU RUH2R
Spacegroup: P21212
Unit cell:
a: 104.7Å b: 174.3Å c: 60.5Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.202 0.202 not available
Expression system: Escherichia coli

Quick links

Citations

29 citation in other articles

Crystal structure of a monocotyledon (maize ZMGlu1) beta-glucosidase and a model of its complex with p-nitrophenyl beta-D-thioglucoside.
Czjzek et al. (2001)
28 more

7 mentions without citation

Structural insights into the substrate specificity of a 6-phospho-β-glucosidase BglA-2 from Streptococcus pneumoniae TIGR4.
Yu et al. (2013)
6 more