› 4pck

X-ray diffraction
2.4Å resolution

Crystal structure of the P22S mutant of N-terminal CS domain of human Shq1

Released:
DOI: 10.2210/pdb4pck/pdb
PDB entry 4pck coloured by chain, front view.
PDB entry 4pck coloured by chain, side view.
PDB entry 4pck coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Structure and interactions of the CS domain of human H/ACA RNP assembly protein Shq1.
Singh M, Wang Z, Cascio D, Feigon J
J Mol Biol 427 807-823 (2015)
PMID: 25553844

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-180163 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Protein SHQ1 homolog Chains: A, B
Molecule details ›
Chains: A, B
Length: 110 amino acids
Theoretical weight: 12.59 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q6PI26 (Residues: 1-96; Coverage: 17%)
Gene name: SHQ1
Sequence domains: SHQ1-like, CS domain
Structure domains: Immunoglobulin-like

Ligands and Environments

1 bound ligand:
Ligand GOL 1 x GOL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 24-ID-C
Spacegroup: P3221
Unit cell:
a: 58.69Å b: 58.69Å c: 146.83Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.23 0.224 0.284
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

3 review citations

Assembly and trafficking of box C/D and H/ACA snoRNPs.
Massenet et al. (2017)
2 more

1 mention without citation

Structure and interactions of the CS domain of human H/ACA RNP assembly protein Shq1.
Singh et al. (2015)