› 4pew

X-ray diffraction
1.51Å resolution

Structure of sacteLam55A from Streptomyces sp. SirexAA-E

Released:
DOI: 10.2210/pdb4pew/pdb
PDB entry 4pew coloured by chain, front view.
PDB entry 4pew coloured by chain, side view.
PDB entry 4pew coloured by chain, top view.
Source organism: Streptomyces sp. SirexAA-E
Primary publication:
Active site and laminarin binding in glycoside hydrolase family 55.
Bianchetti CM, Takasuka TE, Deutsch S, Udell HS, Yik EJ, Bergeman LF, Fox BG
J Biol Chem 290 11819-32 (2015)
PMID: 25752603

Function and Biology Details

Reaction catalysed: 
Successive hydrolysis of beta-D-glucose units from the non-reducing ends of (1->3)-beta-D-glucans, releasing alpha-glucose
Biochemical function:
Biological process:
Cellular component:
Sequence domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-124265 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Exo-beta-1,3-glucanase Chains: A, B
Molecule details ›
Chains: A, B
Length: 561 amino acids
Theoretical weight: 60.32 KDa
Source organism: Streptomyces sp. SirexAA-E
Expression system: Escherichia coli
UniProt:
  • Canonical: G2NFJ9 (Residues: 46-605; Coverage: 100%)
Gene name: SACTE_4363

Ligands and Environments

2 bound ligands:
Ligand MG 3 x MG
Ligand EDO 1 x EDO
1 modified residue:
Ligand MSE 8 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 21-ID-G
Spacegroup: P212121
Unit cell:
a: 53.967Å b: 92.567Å c: 198.304Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.119 0.117 0.16
Expression system: Escherichia coli

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Citations

3 review citations

Identifying and Visualizing Macromolecular Flexibility in Structural Biology.
Palamini et al. (2016)
2 more

3 mentions without citation

Active site and laminarin binding in glycoside hydrolase family 55.
Bianchetti et al. (2015)
2 more