› 4pf9

X-ray diffraction
2.5Å resolution

Function and Biology Details

Reaction catalysed: 
Degradation of insulin, glucagon and other polypeptides. No action on proteins.
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-147045 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Insulin-degrading enzyme Chains: A, B
Molecule details ›
Chains: A, B
Length: 989 amino acids
Theoretical weight: 114.49 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P14735 (Residues: 42-1019; Coverage: 96%)
Gene name: IDE
Sequence domains:
Structure domains: Metalloenzyme, LuxS/M16 peptidase-like

Ligands and Environments

2 bound ligands:
Ligand 2Q6 2 x 2Q6
Ligand ZN 2 x ZN
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 31-ID
Spacegroup: P21
Unit cell:
a: 79.254Å b: 116.419Å c: 124.47Å
α: 90° β: 97.6° γ: 90°
R-values:
R R work R free
0.196 0.195 0.253
Expression system: Escherichia coli

Quick links

Citations

10 review citations

European contribution to the study of ROS: A summary of the findings and prospects for the future from the COST action BM1203 (EU-ROS).
Egea et al. (2017)
9 more

2 mentions without citation

Targeting Insulin-Degrading Enzyme to Treat Type 2 Diabetes Mellitus.
Tang WJ. (2016)
1 more