› 4pfa

X-ray diffraction
2.56Å resolution

Crystal structure of M. tuberculosis in complex with BTO - covalent adduct

Released:
DOI: 10.2210/pdb4pfa/pdb
PDB entry 4pfa coloured by chain, front view.
PDB entry 4pfa coloured by chain, side view.
PDB entry 4pfa coloured by chain, top view.
Source organism: Mycobacterium tuberculosis H37Rv
Entry authors: Neres J, Panda M, Cole S

Function and Biology Details

Reaction catalysed: 
Trans,octacis-decaprenylphospho-beta-D-ribofuranose + FAD = trans,octacis-decaprenylphospho-beta-D-erythro-pentofuranosid-2-ulose + FADH(2)
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-161687 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Decaprenylphosphoryl-beta-D-ribose oxidase Chains: A, B
Molecule details ›
Chains: A, B
Length: 480 amino acids
Theoretical weight: 52.26 KDa
Source organism: Mycobacterium tuberculosis H37Rv
Expression system: Escherichia coli
UniProt:
  • Canonical: P9WJF1 (Residues: 1-461; Coverage: 100%)
Gene names: Rv3790, dprE1
Sequence domains:

Ligands and Environments


Cofactor: Ligand FAD 2 x FAD
1 bound ligand:
Ligand N77 2 x N77
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SLS BEAMLINE X06DA
Spacegroup: P21
Unit cell:
a: 72.856Å b: 82.013Å c: 81.148Å
α: 90° β: 100.77° γ: 90°
R-values:
R R work R free
0.259 0.257 0.307
Expression system: Escherichia coli

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