› 4pfk

X-ray diffraction
2.4Å resolution

PHOSPHOFRUCTOKINASE. STRUCTURE AND CONTROL

Released:
DOI: 10.2210/pdb4pfk/pdb
PDB entry 4pfk coloured by chain, front view.
PDB entry 4pfk coloured by chain, side view.
PDB entry 4pfk coloured by chain, top view.
Source organism: Geobacillus stearothermophilus
Primary publication:
Phosphofructokinase: structure and control.
Evans PR, Farrants GW, Hudson PJ
Philos Trans R Soc Lond B Biol Sci 293 53-62 (1981)
PMID: 6115424

Function and Biology Details

Reaction catalysed: 
ATP + beta-D-fructose 6-phosphate = ADP + beta-D-fructose 1,6-bisphosphate
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
homo tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-132577 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
ATP-dependent 6-phosphofructokinase Chain: A
Molecule details ›
Chain: A
Length: 319 amino acids
Theoretical weight: 34.17 KDa
Source organism: Geobacillus stearothermophilus
Expression system: Not provided
UniProt:
  • Canonical: P00512 (Residues: 1-319; Coverage: 100%)
Gene names: pfk, pfkA
Sequence domains: Phosphofructokinase
Structure domains:

Ligands and Environments

3 bound ligands:
Ligand F6P 1 x F6P
Ligand MG 2 x MG
Ligand ADP 2 x ADP
No modified residues

Experiments and Validation Details

Entry percentile scores
Spacegroup: I222
Unit cell:
a: 122.5Å b: 84.1Å c: 61.5Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.169 not available not available
Expression system: Not provided

Quick links

Citations

14 review citations

Protein-protein interactions: methods for detection and analysis.
Phizicky et al. (1995)
13 more

21 mentions without citation

From Deep Mutational Mapping of Allosteric Protein Landscapes to Deep Learning of Allostery and Hidden Allosteric Sites: Zooming in on "Allosteric Intersection" of Biochemical and Big Data Approaches.
Verkhivker et al. (2023)
20 more