› 4pi9

X-ray diffraction
1.48Å resolution

Crystal structure of S. Aureus Autolysin E in complex with muropeptide NAM-L-ALA-D-iGLU

Released:
DOI: 10.2210/pdb4pi9/pdb
PDB entry 4pi9 coloured by chain, front view.
PDB entry 4pi9 coloured by chain, side view.
PDB entry 4pi9 coloured by chain, top view.
Source organism: Staphylococcus aureus subsp. aureus Mu50
Primary publication:
The mechanism behind the selection of two different cleavage sites in NAG-NAM polymers.
Mihelič M, Vlahoviček-Kahlina K, Renko M, Mesnage S, Doberšek A, Taler-Verčič A, Jakas A, Turk D
IUCrJ 4 185-198 (2017)
PMID: 28250957

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-101960 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Mannosyl-glycoprotein endo-beta-N-acetylglucosamidase-like domain-containing protein Chain: A
Molecule details ›
Chain: A
Length: 228 amino acids
Theoretical weight: 26.07 KDa
Source organism: Staphylococcus aureus subsp. aureus Mu50
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: A0A0H3JT72 (Residues: 35-258; Coverage: 87%)
Gene name: SAV2307
Sequence domains: Mannosyl-glycoprotein endo-beta-N-acetylglucosaminidase

Ligands and Environments

3 bound ligands:
Ligand SO4 3 x SO4
Ligand CL 7 x CL
Ligand 3LT 1 x 3LT
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: BESSY BEAMLINE 14.1
Spacegroup: P212121
Unit cell:
a: 45.626Å b: 69.312Å c: 73.424Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.179 0.177 0.209
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

1 review citation

Catalytic diversity and cell wall binding repeats in the phage-encoded endolysins.
Broendum et al. (2018)
3 other articles

2 mentions without citation

The mechanism behind the selection of two different cleavage sites in NAG-NAM polymers.
Mihelič et al. (2017)
1 more