› 4piw

X-ray diffraction
2.7Å resolution

Crystal structure of sugar aminotransferase WecE from Escherichia coli K-12

Released:
DOI: 10.2210/pdb4piw/pdb
PDB entry 4piw coloured by chain, front view.
PDB entry 4piw coloured by chain, side view.
PDB entry 4piw coloured by chain, top view.
Source organism: Escherichia coli K-12
Entry authors: Wang F, Xu W, Helmich KE, Singh S, Yennamalli RM, Miller MD, Bingman CA, Thorson JS, Phillips Jr GN, Enzyme Discovery for Natural Product Biosynthesis (NatPro)

Function and Biology Details

Reaction catalysed: 
dTDP-4-amino-4,6-dideoxy-alpha-D-galactose + 2-oxoglutarate = dTDP-4-dehydro-6-deoxy-alpha-D-galactose + L-glutamate
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-151085 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
dTDP-4-amino-4,6-dideoxygalactose transaminase Chains: A, B, C, D, E, F, G, H
Molecule details ›
Chains: A, B, C, D, E, F, G, H
Length: 396 amino acids
Theoretical weight: 44.96 KDa
Source organism: Escherichia coli K-12
Expression system: Escherichia coli
UniProt:
  • Canonical: P27833 (Residues: 1-376; Coverage: 100%)
Gene names: JW3765, b3791, rffA, wecE, yifI
Sequence domains: DegT/DnrJ/EryC1/StrS aminotransferase family
Structure domains:

Ligands and Environments

No bound ligands
2 modified residues:
Ligand MSE 104 x MSE
Ligand LLP 8 x LLP

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 21-ID-F
Spacegroup: P2
Unit cell:
a: 125.341Å b: 87.483Å c: 161.554Å
α: 90° β: 91.1° γ: 90°
R-values:
R R work R free
0.237 0.236 0.275
Expression system: Escherichia coli

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Citations

4 mentions without citation

Prediction of homoprotein and heteroprotein complexes by protein docking and template-based modeling: A CASP-CAPRI experiment.
Lensink et al. (2016)
3 more