› 4pj3

X-ray diffraction
2.3Å resolution

Structural insight into the function and evolution of the spliceosomal helicase Aquarius, Structure of Aquarius in complex with AMPPNP

Released:
DOI: 10.2210/pdb4pj3/pdb
PDB entry 4pj3 coloured by chain, front view.
PDB entry 4pj3 coloured by chain, side view.
PDB entry 4pj3 coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
The RNA helicase Aquarius exhibits structural adaptations mediating its recruitment to spliceosomes.
De I, Bessonov S, Hofele R, dos Santos K, Will CL, Urlaub H, Lührmann R, Pena V
Nat Struct Mol Biol 22 138-44 (2015)
PMID: 25599396

Function and Biology Details

Reaction catalysed: 
ATP + H(2)O = ADP + phosphate
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-129933 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
RNA helicase aquarius Chain: A
Molecule details ›
Chain: A
Length: 1475 amino acids
Theoretical weight: 172.56 KDa
Source organism: Homo sapiens
Expression system: Trichoplusia ni
UniProt:
  • Canonical: O60306 (Residues: 19-1485; Coverage: 99%)
Gene names: AQR, KIAA0560
Sequence domains:

Ligands and Environments

2 bound ligands:
Ligand ANP 1 x ANP
Ligand MG 2 x MG
1 modified residue:
Ligand MSE 38 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: BESSY BEAMLINE 14.2
Spacegroup: P212121
Unit cell:
a: 94.986Å b: 140.444Å c: 144.761Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.192 0.191 0.226
Expression system: Trichoplusia ni

Quick links

Citations

12 review citations

R loops: new modulators of genome dynamics and function.
Santos-Pereira et al. (2015)
11 more

2 mentions without citation

Multisystem inflammation and susceptibility to viral infections in human ZNFX1 deficiency.
Vavassori et al. (2021)
1 more