› 4pjm

X-ray diffraction
2.05Å resolution

Myosin VI motor domain in the Pi release state, space group P212121 - soaked with PO4 - located in the active site

Released:
DOI: 10.2210/pdb4pjm/pdb
PDB entry 4pjm coloured by chain, front view.
PDB entry 4pjm coloured by chain, side view.
PDB entry 4pjm coloured by chain, top view.
Source organism: Sus scrofa
Primary publication:
How actin initiates the motor activity of Myosin.
Llinas P, Isabet T, Song L, Ropars V, Zong B, Benisty H, Sirigu S, Morris C, Kikuti C, Safer D, Sweeney HL, Houdusse A
Dev Cell 33 401-12 (2015)
PMID: 25936506

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-123361 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Unconventional myosin-VI Chain: A
Molecule details ›
Chain: A
Length: 788 amino acids
Theoretical weight: 89.85 KDa
Source organism: Sus scrofa
Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: F1RQI7 (Residues: 2-789; Coverage: 62%)
Gene name: MYO6
Sequence domains: Myosin head (motor domain)
Structure domains:

Ligands and Environments

5 bound ligands:
Ligand PO4 1 x PO4
Ligand MG 1 x MG
Ligand ADP 1 x ADP
Ligand GOL 7 x GOL
Ligand IPA 2 x IPA
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID23-2
Spacegroup: P212121
Unit cell:
a: 91.49Å b: 95.33Å c: 103.34Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.162 0.16 0.196
Expression system: Spodoptera frugiperda

Quick links

Citations

16 review citations

How Myosin Generates Force on Actin Filaments.
Houdusse et al. (2016)
15 more

1 mention without citation

How actin initiates the motor activity of Myosin.
Llinas et al. (2015)