› 4pjr

X-ray diffraction
2Å resolution

Crystal structure of designed cPPR-NRE protein

Released:
DOI: 10.2210/pdb4pjr/pdb
PDB entry 4pjr coloured by chain, front view.
PDB entry 4pjr coloured by chain, side view.
PDB entry 4pjr coloured by chain, top view.
Source organism: unidentified
Primary publication:
An artificial PPR scaffold for programmable RNA recognition.
Coquille S, Filipovska A, Chia T, Rajappa L, Lingford JP, Razif MF, Thore S, Rackham O
Nat Commun 5 5729 (2014)
PMID: 25517350

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-165019 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Pentatricopeptide repeat protein Chain: A
Molecule details ›
Chain: A
Length: 303 amino acids
Theoretical weight: 32.92 KDa
Source organism: unidentified
Expression system: Not provided

Ligands and Environments

1 bound ligand:
Ligand MG 3 x MG
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SLS BEAMLINE X06DA
Spacegroup: P212121
Unit cell:
a: 54.28Å b: 74.93Å c: 86.7Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.188 0.185 0.222
Expression system: Not provided

Quick links

Citations

24 review citations

The FASTK family of proteins: emerging regulators of mitochondrial RNA biology.
Jourdain et al. (2017)
23 more

2 mentions without citation

Structural basis for specific single-stranded RNA recognition by designer pentatricopeptide repeat proteins.
Shen et al. (2016)
1 more