› 4pk0

X-ray diffraction
2.3Å resolution

CRYSTAL STRUCTURE OF T4 LYSOZYME-PEPTIDE IN COMPLEX WITH TEICOPLANIN-A2-2

Released:
DOI: 10.2210/pdb4pk0/pdb
PDB entry 4pk0 coloured by chain, front view.
PDB entry 4pk0 coloured by chain, side view.
PDB entry 4pk0 coloured by chain, top view.
Source organisms:
Primary publication:
X-ray crystal structure of teicoplanin A₂-2 bound to a catalytic peptide sequence via the carrier protein strategy.
Han S, Le BV, Hajare HS, Baxter RH, Miller SJ
J Org Chem 79 8550-6 (2014)
PMID: 25147913

Function and Biology Details

Reaction catalysed: 
Hydrolysis of (1->4)-beta-linkages between N-acetylmuramic acid and N-acetyl-D-glucosamine residues in a peptidoglycan and between N-acetyl-D-glucosamine residues in chitodextrins
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-112956 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
TEICOPLANIN-A2-2 Chain: B
Molecule details ›
Chain: B
Length: 7 amino acids
Theoretical weight: 1.21 KDa
Source organism: Actinoplanes teichomyceticus
Endolysin Chain: A
Molecule details ›
Chain: A
Length: 171 amino acids
Theoretical weight: 19.34 KDa
Source organism: Tequatrovirus T4
Expression system: Escherichia coli
UniProt:
  • Canonical: D9IEF7 (Residues: 1-164; Coverage: 100%)
Gene names: T4Tp126, e
Sequence domains: Phage lysozyme
Structure domains: Lysozyme

Ligands and Environments

8 bound ligands:
Ligand GCS 1 x GCS
Ligand NAG 1 x NAG
Ligand MAN 1 x MAN
Ligand T55 1 x T55
Ligand CL 1 x CL
Ligand NA 1 x NA
Ligand PO4 1 x PO4
Ligand GOL 1 x GOL
8 modified residues:
Ligand GHP 3 x GHP
Ligand 3MY 1 x 3MY
Ligand OMY 1 x OMY
Ligand CCS 1 x CCS
Ligand MHS 1 x MHS
Ligand DPR 1 x DPR
Ligand AIB 1 x AIB
Ligand DAL 2 x DAL

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 24-ID-E
Spacegroup: I222
Unit cell:
a: 55.923Å b: 63.233Å c: 138.615Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.192 0.189 0.237
Expression system: Escherichia coli

Quick links

Citations

2 review citations

Late-Stage Diversification of Natural Products.
Hong et al. (2020)
1 more

3 mentions without citation

X-ray crystal structure of teicoplanin A₂-2 bound to a catalytic peptide sequence via the carrier protein strategy.
Han et al. (2014)
2 more