› 4pk2

X-ray diffraction
1.35Å resolution

tubulin acetyltransferase complex with bisubstrate analog

Released:
DOI: 10.2210/pdb4pk2/pdb
PDB entry 4pk2 coloured by chain, front view.
PDB entry 4pk2 coloured by chain, side view.
PDB entry 4pk2 coloured by chain, top view.
Source organisms:
Primary publication:
Molecular basis for age-dependent microtubule acetylation by tubulin acetyltransferase.
Szyk A, Deaconescu AM, Spector J, Goodman B, Valenstein ML, Ziolkowska NE, Kormendi V, Grigorieff N, Roll-Mecak A
Cell 157 1405-1415 (2014)
PMID: 24906155

Function and Biology Details

Reaction catalysed: 
Acetyl-CoA + [alpha-tubulin]-L-lysine = CoA + [alpha-tubulin]-N(6)-acetyl-L-lysine
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-165021 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Alpha-tubulin N-acetyltransferase 1 Chain: A
Molecule details ›
Chain: A
Length: 198 amino acids
Theoretical weight: 22.74 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: Q5SQI0 (Residues: 1-197; Coverage: 47%)
Gene names: ATAT1, C6orf134, MEC17, Nbla00487
Sequence domains: GNAT acetyltransferase, Mec-17
Structure domains: Aminopeptidase
ACETYL-SER-(N-PROPANOYL-LYS)-ASP--THR-NH2 PEPTIDE Chain: B
Molecule details ›
Chain: B
Length: 6 amino acids
Theoretical weight: 530 Da
Source organism: synthetic construct
Expression system: Not provided

Ligands and Environments


Cofactor: Ligand COA 1 x COA
No bound ligands
1 modified residue:
Ligand PRK 1 x PRK

Experiments and Validation Details

Entry percentile scores
X-ray source: ALS BEAMLINE 5.0.1
Spacegroup: P21
Unit cell:
a: 37.124Å b: 52.483Å c: 50.285Å
α: 90° β: 106.83° γ: 90°
R-values:
R R work R free
0.133 0.131 0.168
Expression systems:
  • Escherichia coli
  • Not provided

Quick links

Citations

35 review citations

The tubulin code: molecular components, readout mechanisms, and functions.
Janke C. (2014)
34 more