› 4pl7

X-ray diffraction
2.3Å resolution

Structure of Komagataella pastoris actin-thymosin beta4 hybrid

Released:
DOI: 10.2210/pdb4pl7/pdb
PDB entry 4pl7 coloured by chain, front view.
PDB entry 4pl7 coloured by chain, side view.
PDB entry 4pl7 coloured by chain, top view.
Source organisms:
Primary publication:
Structural basis of thymosin-β4/profilin exchange leading to actin filament polymerization.
Xue B, Leyrat C, Grimes JM, Robinson RC
Proc Natl Acad Sci U S A 111 E4596-605 (2014)
PMID: 25313062

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-238753 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Actin; Thymosin beta-4 Chains: A, B
Molecule details ›
Chains: A, B
Length: 440 amino acids
Theoretical weight: 48.48 KDa
Source organisms: Expression system: Komagataella pastoris
UniProt:
  • Canonical: Q9P4D1 (Residues: 2-376; Coverage: 100%)
  • Canonical: P62328 (Residues: 2-44; Coverage: 98%)
Gene names: ACT1, PAS_chr3_1169, TB4X, THYB4, TMSB4, TMSB4X
Sequence domains:
Structure domains:

Ligands and Environments

2 bound ligands:
Ligand ATP 2 x ATP
Ligand CA 2 x CA
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: NSRRC BEAMLINE BL13B1
Spacegroup: P21
Unit cell:
a: 56.508Å b: 139.495Å c: 56.485Å
α: 90° β: 116.21° γ: 90°
R-values:
R R work R free
0.198 0.195 0.258
Expression system: Komagataella pastoris

Quick links

Citations

6 review citations

Reconsidering an active role for G-actin in cytoskeletal regulation.
Skruber et al. (2018)
5 more

10 mentions without citation

Actin and Actin-Binding Proteins.
Pollard TD. (2016)
9 more