› 4pnp

X-ray diffraction
1.8Å resolution

THE HIGH RESOLUTION CRYSTAL STRUCTURE OF BOVINE SPLEEN PURINE NUCLEOSIDE PHOSPHORYLASE IN COMPLEX FORMS WITH PHOSPHATE AND 9-DEAZAINOSINE

Released:
DOI: 10.2210/pdb4pnp/pdb
PDB entry 4pnp coloured by chain, front view.
PDB entry 4pnp coloured by chain, side view.
PDB entry 4pnp coloured by chain, top view.
Source organism: Bos taurus
Entry authors: Pugmire MJ, Mao C, Ealick SE

Function and Biology Details

Reaction catalysed: 
Purine deoxynucleoside + phosphate = purine + 2'-deoxy-alpha-D-ribose 1-phosphate
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
homo trimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-157333 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Purine nucleoside phosphorylase Chain: A
Molecule details ›
Chain: A
Length: 289 amino acids
Theoretical weight: 32.01 KDa
Source organism: Bos taurus
UniProt:
  • Canonical: P55859 (Residues: 1-289; Coverage: 100%)
Gene names: NP, PNP
Sequence domains: Phosphorylase superfamily
Structure domains: Nucleoside phosphorylase domain

Ligands and Environments

1 bound ligand:
Ligand PO4 1 x PO4
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: CHESS BEAMLINE A1
Spacegroup: P213
Unit cell:
a: 92.72Å b: 92.72Å c: 92.72Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.222 0.222 0.248

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