› 4pqj

X-ray diffraction
1.86Å resolution

Crystal structure of a phosphate binding protein

Released:
DOI: 10.2210/pdb4pqj/pdb
PDB entry 4pqj coloured by chain, front view.
PDB entry 4pqj coloured by chain, side view.
PDB entry 4pqj coloured by chain, top view.
Source organism: Pseudomonas aeruginosa
Entry authors: Opatowsky Y, Neznansky A

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-143644 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Phosphate-binding protein PstS Chains: A, C
Molecule details ›
Chains: A, C
Length: 299 amino acids
Theoretical weight: 32.18 KDa
Source organism: Pseudomonas aeruginosa
Expression system: Escherichia coli
UniProt:
  • Canonical: P0DMR4 (Residues: 25-323; Coverage: 100%)
Gene name: pstS
Sequence domains: PBP superfamily domain
Structure domains: Periplasmic binding protein-like II

Ligands and Environments

1 bound ligand:
Ligand PO4 2 x PO4
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID23-1
Spacegroup: C2221
Unit cell:
a: 67.5Å b: 151.35Å c: 108.98Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.218 0.216 0.262
Expression system: Escherichia coli

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