› 4psc

X-ray diffraction
1.15Å resolution

Structure of cutinase from Trichoderma reesei in its native form.

Released:
DOI: 10.2210/pdb4psc/pdb
PDB entry 4psc coloured by chain, front view.
PDB entry 4psc coloured by chain, side view.
PDB entry 4psc coloured by chain, top view.
Source organism: Trichoderma reesei QM6a
Primary publication:
A Cutinase from Trichoderma reesei with a lid-covered active site and kinetic properties of true lipases.
Roussel A, Amara S, Nyyssölä A, Mateos-Diaz E, Blangy S, Kontkanen H, Westerholm-Parvinen A, Carrière F, Cambillau C
J Mol Biol 426 3757-3772 (2014)
PMID: 25219509

Function and Biology Details

Reaction catalysed: 
Cutin + H(2)O = cutin monomers
Biochemical function:
Biological process:
  • not assigned
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-100128 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Cutinase Chain: A
Molecule details ›
Chain: A
Length: 254 amino acids
Theoretical weight: 26.77 KDa
Source organism: Trichoderma reesei QM6a
Expression system: Trichoderma reesei
UniProt:
  • Canonical: A0A024SC78 (Residues: 45-248; Coverage: 88%)
Gene name: M419DRAFT_76732
Sequence domains: Cutinase
Structure domains: alpha/beta hydrolase

Ligands and Environments

1 bound ligand:
Ligand GOL 1 x GOL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID14-4
Spacegroup: P212121
Unit cell:
a: 29.159Å b: 48.002Å c: 141.579Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.147 0.146 0.167
Expression system: Trichoderma reesei

Quick links

Citations

4 review citations

The Plant Polyester Cutin: Biosynthesis, Structure, and Biological Roles.
Fich et al. (2016)
3 more

5 mentions without citation

Complex lasso: new entangled motifs in proteins.
Niemyska et al. (2016)
4 more